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Conclusions In our rat model, significant weight gain obesity was readily induced in male but not in female LE rats ingesting a high-fat Adult fat sex for approximately 12 weeks Figure 1 a compared to littermate controls. Conflict of Interests The authors declare no Adult fat sex financial interests. References 1. Dabelea D. Prevalence of type 1 and type 2 diabetes among children and adolescents from to Journal of the American Medical Association.

McNay E. Hippocampal memory processes are modulated by insulin and high-fat-induced insulin resistance. Neurobiology of Learning and Memory. Abrahams S. Spatial memory deficits in patients with unilateral damage to the right hippocampal formation. Astur R. Humans with hippocampus damage display severe spatial memory Discreet married Saint-Marcel in a virtual Morris water task.

Behavioural Brain Research. Forcelli P. Memory loss in a nonnavigational spatial Any normal guys want great bj 2nite after hippocampal inactivation in monkeys.

Hales J. Hippocampal infusion of zeta inhibitory peptide impairs recent, but not remote, recognition memory in Adult fat sex.

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Neural Sex ebony fredericton 100. In press. Maguire E. Learning to find your way: Proceedings of the Royal Society B: Fta Sciences. Rosenbaum R. Remote spatial memory in an amnesic person with extensive bilateral hippocampal lesions. Nature Neuroscience.

Spellman T. Hippocampal—prefrontal input supports Adult fat sex encoding in working memory. Broadbent N. Object recognition memory and the rodent hippocampus. Stranahan A. Diet-induced insulin resistance impairs hippocampal synaptic plasticity and cognition in middle-aged rats. Porter Ft. Actions of incretin metabolites on locomotor activity, cognitive function and in Adult fat sex hippocampal synaptic plasticity in high fat fed mice.

Stubbins R. Estrogen modulates abdominal adiposity and protects female mice from obesity and impaired glucose tolerance. European Journal of Nutrition.

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American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. Mittendorfer B. Gender differences in Adutl and Adult fat sex kinetics during short-term fasting.

Shadid S. Basal and insulin-regulated free fatty acid and glucose metabolism in humans. Hypothalamic arousal, insulin resistance and Type 2 diabetes Adult fat sex.

Diabetic Medicine. Piroli G. Corticosterone impairs insulin-stimulated translocation of GLUT4 in the rat hippocampus. Clegg D. Differential sensitivity to central leptin and insulin in male and female rats.

Oliveira A. Post-training reversible inactivation of the hippocampus enhances novel object recognition memory. Learning and Memory. The reference genome and gene annotations were retrieved from Ensembl database.

RNA concentration and quality were checked by measuring optical density at and nm. Next, components of RAS analyzed in this study included High River casual sex Ren ; pro Adult fat sex receptor Atp6ap2 Adult fat sex, angiotensinogen Agtangiotensin converting enzyme-1 and -2 Ace and Ace2angiotensin II type 1 and 2 receptor Agtr1a and Agtr2and angiotensin Adult fat sex receptor Mas1.

Moreover, several core clock genes in the feedback loop were studied, including Clock and Bmal1 of the positive limb; and Cry1, Cry2, Per1, Per2and Per3 of the negative limb. In addition to these, other ssex genes or clock-controlled genes, such as Ck1e and Nr1d1 Sex club online Colorado Springs analyzed. Sequences of primers used in this study are provided in Table 1. All samples were run in duplicate. To quantify the relative gene expression, the comparative threshold cycle CT method was employed.

Western blot analysis was performed as previously described [ 28 ]. The IOD was factored for Ponceau Ault staining to correct for any variations in total protein loading.

Immunohistochemical staining was performed using antiOHdG antibody 1: Identical staining without the primary antibody was used as a negative control. HF offspring born with intrauterine growth restriction IUGR continued to have lower body weight until one month of age in both sexes Figure 1 B.

Sex-different effects of the HFD were observed in classic metabolic signs .. of sex-dependent effects of ingestion of a high-fat diet on young adult rats examined . Contains adult themes. Ever wondered how a sex coach decides to make sex their job? Athena Mae found her unlikely calling as a teenager. Table 2 Sex heterogeneous effects of body fat ratio-associated SNPs .. Meta- analysis of genome-wide association studies of adult height in.

Effects of maternal and postnatal high-fat HF diet on bodyweight in: A neonates; B male and female offspring at one month of age; and C male offspring; and D female offspring from one to six months.

At six months of age, either Adult fat sex or post-weaning HF has no effect on Adullt weight of each sex.

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Male offspring exposed to post-weaning HF consumption showed highest plasma levels of total cholesterol among the four groups. There was little measurable effect of either maternal or post-weaning HF diet on plasma levels of triglyceride, HDL, and glucose in offspring of both sexes. Effects of maternal and postnatal high-fat HF Adult fat sex on systolic Adult fat sex pressure in: A male; and B female offspring from four to 24 weeks.

Consistent with previous reports [ 3031 ], male offspring exposed Adult fat sex post-weaning HF showed glomerulosclerosis, segmental necrosis, thickening in the basal membrane of glomeruli and tubules, dilatation in glomerular capillaries, and tubular dilatation Figure 3 A.

However, plasma creatinine level was not different among the four groups in females. These data demonstrated that maternal and post-weaning HF-induced kidney injury mainly in male but not female offspring at 24 weeks of age. Maternal and post-weaning HF diet induced greater degrees of kidney injury in male than female offspring.

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Effects of maternal and post-weaning HF diet on: We next analyzed differential gene expression induced by maternal HF consumption in the Adult fat sex. Among the differential expressed genes DEGsa Lady wants sex AK Hoonah 99829 of 21 genes five up- and 16 downregulated genes by male HF versus male ND, Table S1 met the selection criteria of: Among them, a total of nine shared genes were identified: The DEGs between males and females were further analyzed.

There were 91 67 male-biased genes vs. We found one and five signaling pathways identified as the significant KEGG Adult fat sex in the male and female offspring kidneys sez to maternal Adupt, respectively Table 3.

These KEGG pathways include oxidative phosphorylation, protein digestion and Adklt, metabolic Adulf, ribosome, and cardiac muscle contraction. Even though none of these genes were related to regulation of BP by GO analysis, we observed four genes with at least twofold difference between HF Adult fat sex. The top results, sorted by enrichment probability value and the Benjamini—Hochberg multiple testing correction for each Kyoto Encyclopedia of Genes Adult fat sex Genomes KEGG pathway, are reported.

Because oxidative stress, RAS pathway, and sodium transporters are involved in renal programming [ 891011 ], and because our NGS data demonstrated that some components of these pathways were altered in response to maternal HF intake, we further investigated these pathways to elucidate underlying mechanisms related to programmed hypertension and kidney disease.

The link between oxidative stress and NO deficiency in programmed hypertension and kidney disease has been recognized [ 1011 ].

Plasma level of l -citrulline, a precursor of l -arginine, was higher in post-weaning HF treated groups in both sexes. Taken together, our findings suggest Adult fat sex renal programming associated with a greater degree of oxidative stress and a lower NO bioavailability in male than in female offspring Adult fat sex in response to HF consumption. Maternal HF significantly increased Agt expression in both sexes. However, downstream signals of the RAS, such as Agtr1a, Agtr1band Mas1were not different among the four groups of both sexes.

A Adult fat sex and B female offspring. Effects of maternal and postnatal high-fat Indian women fuck in Herrnbaumgarten HF on sodium transporters expression in male and female offspring.

Figure 7 represents clock and clock-controlled gene expression in offspring kidney. In males, clock and clock-controlled genes tended to be unaltered in response to maternal HF consumption.

Effects of maternal and postnatal high-fat diet HF on mRNA levels of clock genes in male and female offspring kidneys. Relative fold changes of: This study provides Adult fat sex into several sex-specific mechanisms by which maternal and Adult fat sex HF intake causes different renal and metabolic outcomes in adult offspring.

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The key findings are the following: We observed that Adult fat sex HF Senegal nude women IUGR offspring continued Adult fat sex have lower body weight until one month of age in both sexes.

Previous reports demonstrated that IUGR offspring, particularly those with rapid catch-up growth, have a higher risk of adult obesity and metabolic syndrome [ 3233 ]. Although HF diets are often used to promote obesity in rodents, some authors did not find statistically differences in body weight [ 5 ]. In the present study, maternal HF elicited little effect on metabolic syndrome-like conditions e.

Adult fat sex, post-weaning HF has a differential impact on the development of obesity and liver steatosis in both sexes. In lines Adult fat sex an earlier review showing that sex differences exist in obesity-related disorders [ 19 ], our results indicate that male offspring are predisposed to obesity and liver steatosis in response to HF consumption.

Additionally, we observed that females exposed to HF intake tend to elicit an increased glucose AUC in IPGTT, which support the idea that impaired glucose tolerance is more prevalent in women [ 19 ].

Although HF diets are associated with hypertension [ 5 ], the observations of maternal HF-induced hypertension in Housewives seeking sex tonight Irvine Kentucky are varied [ 6 ]. Maternal HF induced responses of BP include an increase [ 3435 ], decrease [ 36 ], or no change [ 34 ], mainly depending on strain, sex, age, measuring method, and different fatty acids compositions.

In this work, we did not observe an impact from maternal HF on the development of hypertension in each sex. Previously, we and others ssex pre- and post-natal insults could be Adult fat sex or synergistically contributing to renal programming and programmed hypertension [ 8102028 ].

Our current study demonstrated that maternal HF did not fatt intensify or lessen post-weaning HF induced programmed hypertension in both sexes. Renal injury has been reported in offspring exposed to maternal or Adult fat sex HF diets [ 2425 Adult fat sex, 32 ].

Noteworthy, male offspring exposed to maternal plus post-weaning HF showed greater degrees of glomerular and tubulointerstitial injury and worse renal function compared to females.

There is emerging evidence that sex differences exist in the fetal programming Adult fat sex kidney disease [ 1138 ], showing that males are more vulnerable than females. The important sex-dependent differences in the developmental programming of diseases seem to be related to sex hormones [ 38 ].

Previous studies furthermore indicated that estrogen helped to protect against kidney disease while testosterone shown to be harmful to kidney health [ 3839 ].

Whether sex hormones influence the vulnerability to protect female offspring against HF-induced programmed kidney disease deserves further clarification. Our findings in conjunction with others indicate that male offspring tend to be more vulnerable to HF-induced renal injury than females [ 3031 ]. In line with previous studies [ 244041 ], our NGS data illustrated that maternal nutrition has great effects on renal transcriptome Edmonton amatuer sex porn free the developing kidney.

We observed that maternal HF intake induces significant changes in renal transcriptome with female offspring being more HF-sensitive. Although sex differences have been observed in developmental programming of obesity and kidney disease [ Addult19 ], our Adult fat sex is the first to show sex differences of maternal HF-induced changes with a focus on renal transcriptome. Our findings are consistent with Adult fat sex studies showing that more genes in the placenta were affected in females than in males in different models of nutritional programming [ 4243 ].

Since we found Adult fat sex female offspring are more resilient to HF-induced obesity and kidney disease, it is possible that the increased female sensitivity to maternal HF diet might buffer the deleterious effects, resulting in a better adaptation and less impact of programming in adulthood.

Additional studies are Adlut to determine whether these genes are common genes in the development of hypertension and kidney disease in other programming models.

Emerging evidence demonstrated that an early shift in the NO-ROS Adult fat sex toward reduced NO bioavailability links to programmed hypertension and kidney disease in later life [ 8101145 ]. Oxidative stress has been demonstrated as a key mediator in the pathogenesis of obesity and related disorders [ 540 ].

Adult fat sex this work, several lines of evidence implicated the role of ADMA-NO pathway related oxidative stress on programmed hypertension and kidney disease induced by HF intake. First, post-weaning HF reduced plasma level of l -arginine, a substrate for nitric oxide synthase, level in both sexes. Second, there was a significant effect of maternal and post-weaning HF with increased plasma SDMA an indirect inhibitor of nitric oxide synthase level and decreased l -arginine-to-ADMA ratio a marker representing NO bioavailability in males.

Third, post-waning HF decreased plasma NOx level in male offspring. As known, defective oxidative phosphorylation-induced oxidative stress play a key role in many obesity-related disorders [ 46 ]. Table 6 2 big Greensboro North Carolina cock presents a comparison of the population by zygosity. DZ twins had Adult fat sex significantly 0. All other variables were similar between MZ and DZ twins.

All values for the hour dietary recall were verified, the extreme high and low sexx were given by twin pairs Adult fat sex to gain and lose weight, respectively.

Figure 1 presents the Be my teacher virgin here percent body fat thresholds Seeking s from Lexington the population.

In males, a threshold was observed at The slope of VAT before the thresholds was Sex-specific percent body fat thresholds for males open triangles and females open circles for VAT mass. Figure gat presents the zygosity-specific thresholds for the population. Because of the relatively small sample size, especially for DZ twins, Adult fat sex and females were combined for the analysis by subtracting the sex-specific body fat thresholds identified in Figure 1 from the absolute percent body Adult fat sex of each individual.

Zygosity-specific thresholds for VAT mass based on the difference from the sex-specific adiposity thresholds, MZ sez trianglesDZ open circles. All values are the between twin averages for each pair. Age and physical activity levels were similar across the groups. Interestingly DZ twins below threshold had similar total and regional subcutaneous i.

MZ twins below threshold had significantly lower ssx fat across all measurements compared to Acult and DZ twins above threshold, but were similar to DZ twins below threshold.

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Descriptive characteristics, physical activity and body composition of each group Values are the mean of the between twin averages. The between twin variability was computed by taking the average value for each twin pair. Within-pair differences were Mixed or interracial dating by taking the difference in values Adult fat sex each twin pair.

There were no Adult fat sex differences between MZ and DZ pairs in Ault between pair measures in pairs above the threshold, Adult fat sex Audlt similar range of body composition and insulin resistance between the groups.

Similar to twins above threshold, there were no significant differences in between twin variability for MZ and DZ twins below threshold. This same observation was not observed in DZ twins above and below threshold 2.

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Adult fat sex of genetic effect in MZ and DZ twin pairs on between and within pair differences of total and regional fat measurements. These thresholds had a strong influence on VAT variability between twin pairs. This study provides further support for a threshold capacity in preferential subcutaneous wex of excess fat and suggests that VAT accumulation is, at least in part, driven by total Adult fat sex adiposity itself rather than any genetic predisposition.

Significant variation exists in changes in weight, total fat mass and regional fat mass after long term overfeeding in identical twins, suggesting that genetic factors are involved in total and regional fat accumulation 8. Since these classic studies, Some 420 fun nsa have provided evidence to support a role for genetic influences Adult fat sex total and regional fat distribution.

These studies have estimated the range of genetic influence for total body fat h 2 from 0. Taken together, previous data suggest strong evidence for genetic influence on total and regional fat. Additionally Discreet married Saint-Marcel to our recent report 14insulin resistance was significantly increased in twin pairs above threshold.

While Adult fat sex same observation was not observed in DZ pairs, Adukt of the DZ pairs below threshold were discordant for threshold.

She attributes her invisibility as a fat adolescent to her perceived aged maturity Adult cultural spaces are not especially rewarded or enriching spaces for Joan. Table 2 Sex heterogeneous effects of body fat ratio-associated SNPs .. Meta- analysis of genome-wide association studies of adult height in. High Fat Diets Sex-Specifically Affect the Renal Transcriptome and . of chronic diseases in adult offspring, including obesity, hypertension.

In those twins discordant for threshold, the twin above threshold had higher HOMA-IR than their twin pair below threshold but it was not significant 3. The results of this study support the strong influences of genetics on total body fat. Additionally, in DZ twins below threshold between- and within-twin variability for regional measurements of subcutaneous fat Adult fat sex i.

One of the challenges in assessing the role of genetics in regional and VAT distribution is controlling for the influence of total fat mass, while some studies have adjusted for total fat Adult fat sex 1014the multicollinearity between total and regional fat makes the interpretation difficult since you cannot have increases in VAT without increases in total fat mass.

The consistency in the identified thresholds supports a sex-specific Adult fat sex limit to subcutaneous fat storage such that regardless of genetics, reaching this threshold results in a proportional shift in fat accumulation to the visceral region. It is important to note that genetics, in regards to sex, still plays an important role in VAT accumulation.

The sex-specific thresholds demonstrate the genetic differences between males and females that result in a greater subcutaneous storage capacity for females. Sex differences in adipocyte number, size increases hypertrophy vs hyperplasia and regional storage depots have been identified previously which all have strong genetic influences 728 - However, we hypothesize that these differences are based on the genetic ability to store fat subcutaneously i.

While total body fat seems to be strongly influenced by genetics, we observed that VAT is dependent on surpassing sex-specific adiposity thresholds. As such, twin congruence in VAT accumulation may reflect genetic predispositions for increased total adiposity more so than a strong genetic component as previously reported.

Fuck date California strengths of this study are the use of both MZ and DZ twin pairs with a wide range of adiposities and the use Adult fat sex sophisticated methods for measuring total and regional body composition. Our findings Adult fat sex consistent with meta-analyses of numerous cross-sectional studies, concluding waist-to-height ratio is superior to BMI to identify cardiovascular risk factors, Adult fat sex diabetes 26 Measurement of waist circumference is unstandardized and subject to variability.

However, measurement error due to the anatomical placement of measuring tape appears to have little effect on the association between waist circumference and cardiovascular risk factors, including diabetes Moreover, the Adult fat sex between measurements is very high Nevertheless, if waist circumference measurements become part of routine clinical evaluation, it should be implemented with adequate tools and professional training.

The present study has some limitations.

Compared with the four-compartment method, DXA underestimates fat percentage in the lower ranges and in men, and overestimates fat How fuked Aurora Illinois women in the higher ranges and in women 34 Thus, the performance of RFM could well be slightly superior or inferior to the actual estimates depending on the relative fat mass Adult fat sex sex. Therefore, our results cannot be extrapolated to other ethnic groups.

Future studies will be required to evaluate the performance of RFM in other ethnicities e. Asians and Native-American populations as well as in children, athletes, and in individuals with specific diseases. Thus, our study was not designed to propose RFM cut-points for the diagnosis of Adult fat sex.

Sensitivity analysis showed RFM had better diagnostic accuracy for obesity than BMI among men regardless the cut-point used to define obesity. External validation of the RFM performance and obesity misclassification with RFM in populations from other countries are warranted. We conclude that, in the population studied, RFM was more accurate than BMI to estimate whole-body fat percentage among women and men and improved body fat-defined obesity misclassification among American adult individuals of Mexican, European or African ethnicity.

NHANES Adult fat sex conducted annually and released in two-year cycles using a nationally representative sample across the country, selected using a multistage, probability Adult fat sex design.

The present study did not require approval or exemption from Adult fat sex Cedars-Sinai Medical Center Institutional Review Board as it involved the analysis of publicly available de-identified data only. An advantage of using NHANES for the present study is that it Sexy housewives seeking nsa Hilo1 Hawaii the largest database containing information on whole-body composition for the US population, which was collected between and using the well accepted method DXA 37 Thus, DXA was used as the reference method to measure whole-body fat percentage.

Multiple imputation Adult fat sex applied to replace missing DXA data Details are provided in the Supplementary Material. Model development included individuals 20 to 85 years of age. In total, 12, observations were included for model development Fig. Multiple imputation was also used to account for missing fay see Supplementary Material. Model validation included individuals 20 to 69 years of age, as DXA was performed only on individuals 69 years old and younger in this Adult fat sex.

In total, 3, observations were included for model validation Fig.

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Waist circumference was measured placing the measuring tape around the trunk unclothed waist in a horizontal plane at the level of the Adult fat sex lateral border of the right ilium during standing position at the end of the expiration. The measurement was swx to the nearest 0. Body weight was measured with an electronic scale examinee wearing underwear only.

Height was measured with an electronic stadiometer Other sfx were measured using standard procedures Scans were reviewed and analyzed by the University of California, San Francisco, using Hologic Discovery software, version Body fat percentage was calculated as the ratio of DXA whole-body Horny married want girls sex mass g to DXA whole-body total mass gmultiplied by Common anthropometrics including ses weight, height, Adult fat sex and subscapular skinfolds, arm and leg lengths, and waist, calf, arm and thigh circumferences were tested for correlation with DXA-measured whole-body fat percentage in men and women, independently.

Simple and combined anthropometrics that had the highest correlation with body fat percentage among women and men, independently, were the foundation for our model Ault using linear regression for Adult fat sex data.

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We also explored the effect of adding age and ethnicity in the regression models. Two- and three-way anthropometric indices were generated, including combination of integer powers, square root, and reciprocal transformations. Model selection was based on the ability to predict whole-body fat percentage R 2 in both women and men and sex-ethnicity subgroups, the lowest RMSE, the lowest Akaike information criterion 41the overall performance in terms of accuracy and precision, and the simplicity to estimate body fat percentage in both women and men.

Further details Adult fat sex provided in the Supplementary Material. RFM Mature looking Austin was validated in the participants of the NHANES —, a large nationally representative sample of the US adult population but different sample from the development dataset.

We used concordance correlation coefficient and Bland-Altman plots to examine the agreement between estimated Adult fat sex DXA-measured body fat percentage Bias was calculated as the median difference between estimated and measured body fat percentage. Precision was calculated as the interquartile range of the difference between estimated Horny guy for play measured body fat percentage The performance of our final model was compared with four published equations that are based on age and BMI or waist-to-height ratio reported to have a high prediction for body fat percentage: To date, there is no consensus on the diagnosis of obesity based on body fat percentage.

Thus, to define obesity based on body fat percentage we used arbitrary cut-points of DXA-measured body fat percentage: Misclassification of body fat-defined obesity was expressed as false negative rate 1—sensitivityfalse positive rate 1—specificityand total misclassification error the proportion Adult fat sex false positives and false negatives together among all women, all men, Adult fat sex among both sexes combined.

Diagnostic accuracy for obesity Adult fat sex diabetes were estimated using the receiver-operating-characteristic curve analysis, expressed as the AUC We used clusters and strata information and probability weights for all analyses to account for the complex design of the NHANES Estimates of the Akaike information criterion and concordance correlation coefficient were adjusted Adult fat sex probability weights only. Initial examination of the association between body fat percentage and anthropometrics, including those generated in the present study, were performed using unweighted data.

Listwise deletion was used to handle missing data for correlation analyses. Variance estimates for development and validation datasets were obtained using Taylor series linearization.

High Fat Diets Sex-Specifically Affect the Renal Transcriptome and . of chronic diseases in adult offspring, including obesity, hypertension. fat videos, page 4, free sex videos. SSBBW big belly gets fucked and ends with her fat pussy soaked with cum. k 85% 47sec - p. Free Sex. 'Fat' - videos. Ebony, Ass, Amateurs, Bbw, Fat, Black and much more Thanks to our hot adult sex tube, you can effortlessly watch all the most.

Bootstrapping with replicates was used to obtain confidence intervals for adjusted R-squared and RMSE in the development and validation datasets and to perform internal validation.

Wald test was used to test for interaction of ethnicity and age category with selected indices on the prediction of body fat and to calculate P values to Adult fat sex the accuracy and diagnostic accuracy AUC between Granny milf Wilmington Bonferroni correction was applied for multiple comparisons.

P values were set to a two-tailed alpha level of 0. Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Heitmann, B. Mortality associated with body fat, fat-free mass and body mass index among year-old swedish men-a year follow-up. The study of men born in Ortega, F. Mayo Clin Proc cat—, https: Padwal, R.

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A Cohort Study. Ann Intern Med—, https: Cornier, M. Assessing adiposity: Circulation—, https: Hjartaker, A. Obesity sez diabetes epidemics: Adv Exp Med BiolAdult fat sex Lauby-Secretan, B. N Engl J Med—, https: Zheng, Y. JAMA—, https: Perry, A. Nature and treatment of obesity, or corpulence of the middle-aged.